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Table 2 Observed mutational differences between E. coli strains FBR5 and MG1655a

From: A Recurrent Silent Mutation Implicates fecA in Ethanol Tolerance by Escherichia coli

Function

Gene

Mutation

Description

Notes

Ethanol pathway

ldhA

insertion ca. T152

D-lactate dehydrogenase

b,c

pflB

5′ del up to S397

Pyruvate formate lyase

b

Cellular respiration

dcuS

T198I

Histidine protein kinase; regulates anaerobic fumarate metabolism in response to extracellular fumarate concentrations

 

Cell wall

mltA

S268G

Transglycosylation of the muramic acid residue

 

Membrane transport

focA

del

Membrane protein; formate export

b

gcvB

insertion ~ nt 2,940,787

sRNA that represses oppA, dppA, gltI and livJ

d

Metabolic functions

gatY

insertion ca. E149

Encodes tagatose-bisphosphate aldolase

c

Protein synthesis

ycaO

3′ del starting at A565

Ribosomal protein S12 methylthiotransferase accessory factor

 

Transcription

rpoA

L300F

RNA polymerase alpha subunit

 

rpoS

Q33stop

RNA polymerase sigma subunit

e,f

Pseudogenes

intQ

F261 L

  

Unknown function

ylbE

E39E

Domain of unknown function 1116 family member; induced by NO

 

yphD

possible insertion ~ V236

ABC transporter of unknown function

 

Insertion sequences, transposons, and repetitive elements

RIP321

CG insertion at nt 4,294,403

Repetitive element

 

Mutations outside of known genes

 

possible insertion ~ nt 2,985,196

between yqeG (putative hydroxy/aromatic amino acid permease) and yqeH (predicted LuxR family transcriptional regulator)

 
  

C → T at nt 3,957,957

between ppiC (peptidylprolyl-cis-trans isomerase; protein folding) and yifO (hypothetical conserved protein)

 
  1. aGenes previously implicated in ethanol tolerance are in boldface type. Abbreviations: del deletion, fs frameshift, nt nucleotide
  2. bFBR5 carries disruptions of these genes as a consequence of its derivation from E. coli strain NZN111 [27]
  3. cPreviously implicated in ethanol stress response in [28]
  4. dOppA and several dpp genes (although not dppA) have been previously implicated in ethanol stress response in [28]
  5. ePreviously implicated in ethanol tolerance in [29]
  6. fPreviously implicated in alcohol tolerance in [30]