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Table 3 Mutations in DnaA-boxes and ORFs of the MiniR1–1 plasmid

From: Mutations of DnaA-boxes in the oriR region increase replication frequency of the MiniR1–1 plasmid

Plasmid

Location of mutation

Sequence

  

Before mutagenesis

After mutagenesis

pbox1CA-TG

DnaA-box1

TTATCCACA

TTATCtgCA

(DnaA-box1a)

pbox1TT-GG

DnaA-box1a

TTATCtgCA

ggATCtgCA

pbox3TA-GG

DnaA-box3

TTATACATG

TTAggCATG

pORF2M-V

DnaA-box4

TTATGCTGA

TTgTGCTGA

pbox8TT-GG

DnaA-box8

TCTTACACA

TCggACACA

pORF111L-W

ORF1

TTGATT

TgGATT

  1. Mutations in DnaA-boxes and ORFs of the MiniR1–1 plasmid were introduced using primers listed in Table 2 and the ‘Easy mutagenesis system’ from Transgen biotech. Positions of the mutations and sequences before and after mutagenesis are listed. Lowercase letters indicate changed nucleotides
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BMC Microbiology

ISSN: 1471-2180

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