Table 1 Strains and plasmids
From: Mutations of DnaA-boxes in the oriR region increase replication frequency of the MiniR1–1 plasmid
Strain or plasmid | Genotype | Reference |
|---|---|---|
MG1655 | Wild-type | [36] |
MOR70 | MG1655/MiniR1–1 | [31] |
MOR72 | MG1655/pACYC177 | [31] |
MiniR1–1 | oriR1, bla, repA, tap, copA | This work |
BTH101 | F cya-99 araD139 galE15 galK16 rpsL1 (Strr) hsdR2 mcrA1 mcrB1 | [35] |
pKNT25 | repp15AKmR placT25(pSU40 derivative) | [37] |
pUT18 | repColE1ApR placT18(pUC19 derivative) | [37] |
pKNT-dnaA | dnaA fused to T25 on pKNT25 | This work |
pUT-repA | repA fused to T18 on pUT18 | This work |
pMOR1 | MiniR1–1-datA | [31] |