Fig. 4

Mutations in UgtP’s putative hexose-binding site enhance susceptibility to proteolysis in vivo. (a) A schematic representation of YFP-UgtP localization and cell size for wild type, uracil-binding (URA-), hexose-binding (HEX-), and self-oligomerization (OLI-) mutants in either LB or minimal sorbitol (Additional file 3:Figure S3 & [20]). UgtP-His variants BH736 (WT), BH742 (URA-), BH752 (HEX-), BH740 (OLI-) were cultured in either (b) LB + 0.5% xylose or (c) minimal sorbitol + 0.5% xylose and subjected to semi-quantitative immunoblotting against His and FtsZ (loading control) antibodies. Relative expression compared to WT (BH736) UgtP-His is shown (n = 3, error = SD)