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Fig. 2 | BMC Microbiology

Fig. 2

From: Detection and genotyping of Helicobacter pylori in saliva versus stool samples from asymptomatic individuals in Northeastern Thailand reveals intra-host tissue-specific H. pylori subtypes

Fig. 2

Detection of H. pylori directly from stool samples using IFA, semi-nested PCR targeting vacA and real-time PCR targeting 16S rRNA. (A) Fluorescent photomicrograph of H. pylori in various concentrations (CFUs/0.1 g), NC and PC refers to negative (sterile Brucella broth) and positive control (H. pylori DMST 20165 in Brucella broth), respectively. (B) Semi-nested PCR targeting vacA in spiked stool samples. Lane M, 1 kb DNA ladder; Lane 1, negative control (PCR reagent without DNA); Lane 2, positive control (DNA sample from H. pylori DMST20165); Lanes 3–11, stool samples spiked with various numbers of H. pylori cells (108–100 CFUs/0.1 g). (C) Amplification curve of SYBR green real-time PCR targeting 16S rRNA in spiked stool samples. Black line, positive control (DNA sample from H. pylori DMST20165); Red line, 106; orange line, 104; blue line, 103; purple line, 101 CFUs/0.1 g; grey line, Non-template control (PCR reagent without DNA)

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