Fig. 3

Examples of visualizations produced by the PHENOS software. The colour bars and some of the labels have been enlarged for this publication. a Agar thickness of an unprinted plate, showing a low-quality plate with a bulge due to inadequately mixed media. For a good quality plate, all squares would be the same colour (the colour scale shows absorbance at each location in the array-darker colours signifying thicker agar-and is comparable between different visualizations). There should be no pattern to the size of squares either: the largest and smallest squares are the highest and lowest readings on that plate respectively. Squares are also labelled with the array position, although those labels are not legible in these small reproductions. b Printing quality, based on initial readings immediately after printing, with agar subtracted. As with A, the colour scale is absolute and comparable between different experiments, but the spot scaling shows where the largest and smallest printed masses on the plate are located. Each spot is labelled with the strain name, which is legible on full size images. c Final measurements of a plate as measured by the microplate reader (radius being proportional to OD₆₀₀ with agar absorbance subtracted), next to (d) an actual image of the same plate. In this case, there are four replicates of each strain. e Control growth curves for all 384 colonies on a YPD plate, coloured by the initial printed mass as described for B. In a successful treatment experiment such as is shown in C & D, there would be much greater diversity in these curves. f Replicate plots, showing growth curves of all four replicates of a single strain for which growth has been equally suppressed (compare to E) by hydroxyurea. Dots on these curves indicate where the PHENOS software has calculated the inflection point of each curve to lie, i.e. the point of maximum growth. Curves are also labelled with the array position of the colony