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Fig. 2 | BMC Microbiology

Fig. 2

From: Phosphate signaling through alternate conformations of the PstSCAB phosphate transporter

Fig. 2

Pi-signaling in wild type cells and experimental strains. a AP activity levels for BW25113 (wt) and BW26337 (ΔpstSCABphoU) with pRR48, p48SCABU, p48SCAB(Q160K)U or p48SCAB(E179Q)U plasmids. Cells were grown overnight in either MOPS LoPi or MOPS HiPi media, diluted in the morning and grown for an additional seven hours in the indicated medium. Bacterial AP activities were calculated from the averages of three biological replicates performed in duplicate. Error bars represent ± standard error of the mean. **, P < 0.01 for the null hypothesis that the means are the same for the AP units between the strain harboring the p48SCABU plasmid grown in either low or high Pi medium and the mutant strains grown in the same medium, as determined by a two-tailed Student t test. b Western blot to confirm protein stability. The indicated plasmids were introduced into BW26388, a strain that contains a ΔpstB::kan deletion mutation, and the strains were grown overnight in MOPS HiPi medium containing 50 μM IPTG and 50 μg/ml ampicillin and processed for Western blotting

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