Molecular detection of Porcine circovirus type 2 in swine herds of Eastern Cape Province South Africa

BMC Microbiology

Table 3 Primer pairs used for conventional PCR screening and subsequent nucleotide sequencing of partial PCV2 genomes

Primer identity	Primer sequence	Amplicon length	Nucleotide Position	Reference
P1Fw	5′-TAATCCTTCCGAAGACGAGC-3′	629	116–135	[27]
P2Rv	5′-CGATCACACAGTCTCAGTAG-3′	629	726–745	[27]
P3Fw	5′-CAGAAGCGTGATTGGAAGAC-3′	630	531–550	[27]
P4Rv	5′-ATGTAGACCACGTAGGCCTC-3′	630	1142–1161	[27]

ISSN: 1471-2180