Table 1 Strains and plasmids used in this study
From: Regulation of bacteria population behaviors by AI-2 “consumer cells” and “supplier cells”
Strains and plasmids | Gene types and characteristics | Source |
|---|---|---|
Strains | ||
E. coli MG 1655 | F-φ80 lac ZΔM15 Δ(lacZYA-arg F) U169 endA1 recA1 hsdR17(rk − ,mk +) supE44λ- thi − 1 gyrA96 relA1 phoA | Transgene |
E. coli NK-C1 | MG1655 contains pLsrACDB | This study |
E. coli NK-C2 | MG1655 contains pLsrACDBK | This study |
E. coli NK-C3 | MG1655 contains pLsrACDBFG | This study |
E. coli NK-C4 | MG1655 contains pLsrACDBFGK | This study |
E. coli SU1 | MG1655 contains pLuxS | This study |
E. coli SU2 | MG1655 contains pLuxS-Mtn | This study |
Plasmids | ||
pTrcHisB | Cloning vector Apr | |
pLsrACDB | pTrcHisB derivative, containing lsrACDB | This study |
pLsrACDBFG | pTrcHisB derivative, containing lsrACDB, and lsrFG | This study |
pLsrACDBK | pTrcHisB derivative, containing lsrACDB and lsrK | This study |
plsrACDBFGK | pTrcHisB derivative, containing lsrACDB,lsrFG,lsrK | This study |
pLuxS | pTrcHisB derivative, containing luxS | This study |
pLuxS-Mtn pLGFP | pTrcHisB derivative, containing luxS and mtn pTrcHisB derivative, containing lsr promoter and gfp | This study This study |