Fig. 4

Absolute quantification of CpxAR, CpxP, and ArcBA under various conditions. A) Selected reaction monitoring (SRM) was used to determine the absolute amounts of CpxA, CpxR, CpxP, ArcB, and ArcA after addition of 5 μg ml⁻¹ gentamicin (red bars) and after nlpE overexpression (Cpx-ON; green bars). All depicted values represent ratios between the condition of interest and WT cells. Shown are mean data and standard deviations of five biological replicates measured. Values labeled with * represent molecular amounts published in a previous study [34] but are listed here for complete information. B) The absolute amounts of CpxA, CpxR, ArcA, and ArcB were used to calculate the SK:RR-ratio for each tested condition