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Table 1 Characterization of mAbs reactive to the epitopes within Omp31 of B. melitensis

From: Characterization of novel Omp31 antigenic epitopes of Brucella melitensis by monoclonal antibodies

MAb

Isotype

Peptide ELISA

rOMP31 Western-Blot

NMP Western-Blot

NMP

ELISA

SSP

ELISA

TC

IFS

B. m

ICS

Epitope (aa)/peptide

1H2

IgG1 (K)

12

++

−

<1

1

+

−

L (33–48/42–57)/P05/06

2D2

IgM (K)

7

++

++

4.5

4.8

+

++

L (33–48/42–57)/P05/06

2G9

IgG1 (K)

3

++

++

<1

2.2

+

+

L (33–48/42–57)/P05/06

7A3

IgG1 (K)

1.2

++

++

7

17

+

+

L (33–48/42–57)/P05/06

5B1

IgG2a (K)

3.8

+

−

1.2

2

+

++

L (87–102)/P11

2C1

IgG2a (K)

2.7

++

++

2.4

5.5

++

++

L (159–174/168–183)/P19/20

2E7

IgG2a (K)

1.8

++

++

1.5

3.5

++

±

L (159–174/168–183)/P19/20

4E9

IgG2a (K)

3.7

+

++

2

5

++

++

L (159–174/168–183)/P19/20

4H10

IgG2a (K)

1.9

++

++

2.2

4.2

++

−

L (159–174/168–183)/P19/20

8F11

IgG2a (K)

2.2

++

++

2.5

5.5

++

+

L (159–174/168–183)/P19/20

2A8

IgG1 (K)

7.8

++

+

<1

1.5

(+)

+

L (177–192)/P21

6D8

IgG1 (K)

4.7

++

−

<1

<1

+

±

L (177–192)/P21

5B3

IgG2a (K)

2.8

++

+

<1

16

+

++

L (204–219)/P24

2B6

IgM (K)

<1

++

−

8

11

−

+

SC(20–240)

4H6

IgM (K)

<1

++

−

<1

1.2

−

±

SC(20–240)

5B11

IgM (K)

<1

++

−

5.8

9.2

−

±

SC(20–240)

5H3

IgG2a (K)

<1

++

++

11

18

+

++

SC(20–240)

6E10

IgG2a (K)

<1

+

+

1

2

++

−

SC(20–240)

6F9

IgG2a (K)

<1

+

++

<1

1.5

++

±

SC(20–240)

11C4

IgG2a (K)

<1

++

++

1.2

2.6

++

±

SC(20–240)

2H5

IgM (K)

<1

−

−

4.1

7

−

−

C (20–240)

5F11

IgM (K)

<1

−

−

8.2

12

−

+

C (20–240)

  1. NMP, native membrane proteins extracts of B. melitensis; SSP, supernatant of sonicated B. melitensis proteins; TC IFS, Lentivirus-mediated Omp31, transduced 293Tcells detected by immunofluorescent staining; B.m ICS, B. melitensis strain detected by immunochemical staining; L, linear; SC, semi-conformational; C, conformational. The numbers indicate the S/CO values in the EIA; the reactivity levels are indicated by ++ (strongly reactive), + (reactive), ± (indeterminate) or – (non-reactive) in the Western-blot, IFS and ICS. (+), indicate false positive for the negative control of cells. Five IgG and three IgM clones in bold are representatives of high capacity of mAbs reacting with native Omp31 antigens of B. melitensis in various assays.