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Table 3 Comparison of qPCR and conventional isolation of Enterococcus cecorum

From: Colonization patterns of Enterococcus cecorum in two different broiler production cycles detected with a newly developed quantitative real-time PCR

Cycle

dpha

broiler ID

Sample

40-Ct

Conventional isolation

I

31

1

Femoral head

0.3

-

I

31

1

Free thoracic vertebra

17.0

+

I

31

2

Femoral head

1.0

-

I

31

2

Free thoracic vertebra

7.7

-

I

31

3

Femoral head

0.3

-

I

31

3

Free thoracic vertebra

0.0

-

I

31

4

Femoral head

2.5

-

I

31

4

Free thoracic vertebra

2.6

-

I

31

5

Femoral head

5.4

-

I

31

5

Free thoracic vertebra

20.3

+

I

31

6

Femoral head

4.8

-

I

31

6

Free thoracic vertebra

8.2

-

I

31

7

Femoral head

2.8

-

I

31

7

Free thoracic vertebra

17.4

+

I

31

8

Femoral head

0.0

-

I

31

8

Free thoracic vertebra

17.4

+

II

32

1

Cecum

21.3

+

II

32

2

Cecum

21.4

-

II

32

3

Cecum

11.2

-

II

32

4

Cecum

16.5

+

II

32

5

Cecum

15.3

-

II

32

6

Cecum

7.1

-

II

32

7

Cecum

8.9

-

II

32

8

Cecum

7.2

-

II

32

9

Cecum

6.2

-

II

32

10

Cecum

11.2

-

  1. aDays post hatch