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Table 5 Multiplex assay accuracy for CHIKV and DENV

From: Simultaneous detection of chikungunya virus, dengue virus and human pathogenic Leptospira genomes using a multiplex TaqMan® assay

EQA ID

Our laboratory multiplex assay

Ct

Reference laboratory assay

Ct

All enrolled laboratory in SEGA network

Ct

Bias against reference laboratory

Bias against all enrolled laboratories in SEGA network

CHIKV

 EQA-SEGA-05-01

23.98

23.19

26.53

+3.41%

-9.61%

 EQA-SEGA-05-05

25.04

26.50

26.70

- 5.51%

-6.22%

 EQA-SEGA-05-08

22.94

22.06

24.93

+3.99%

−7.98%

 Mean

23.99

23.92

26.05

+0.29%

-7.91%

DENV

 EQA-SEGA-05-03

27.51

30.13

28.28

−8.70%

-2.72%

 EQA-SEGA-05-04

33.97

36.57

33.23

−7.11%

+2.23%

 EQA-SEGA-05-06

26.56

28.56

26.68

−7.00%

-0.45%

 EQA-SEGA-05-09

30.26

33.39

30.58

−9.37%

−1.05%

 Mean

29.57

32.16

29.69

−8.05%

−0.40%

  1. A negative bias against reference laboratory accounts for better performance of our multiplex assay