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Table 3 Some phenotypic characteristics of the studied isolates

From: Identification and sequence analyses of novel lipase encoding novel thermophillic bacilli isolated from Armenian geothermal springs

Phenotypic characteristics

Akhurik 107

Tatev

65–4

Karvachar

QB2

B. subtilis ATCC6051

G. toebii SK-1

A. flavithermus DSM 2641

Cell size (μm)

      

width

1.0–2.0

1.2

1.0

0.7–0.8

0.5–0.9

0.8–0.85

length

4.0–5.0

4.25

1.8

2–3

2–3.5

2.3–7.1

Endospore

      

form

Ellipsoidal

Ellipsoidal

Ellipsoidal

Ellipsoidal

Ellipsoidal

Ellipsoidal

location

Central

Sub-terminal

Terminal

Central

Sub-terminal to terminal

Terminal

Swell sporangia

+

+

+

+

Temperature range (optimum) (°C)

30–60 (55)

45–70 (65)

40–70 (60)

20–55 (28–30)

45–70 (60)

30–72 (60–65)

pH range (optimum)

5–10 (7–8)

6–9 (7)

7–11 (9–10)

5.5–8.5 (7)

6–9 (7.5)

5.5–9 (7)

NaCl range (%)

≤5

≤2.5

≤2

≤7

≤5

≤2.5

Voges-Proskauer test

+

+

+

+

Acid formation from

      

D-glucose

+

+

+

+

Xylose

+

+

Mannitol

+

+

+

Nitrate reduction to nitrite

+

+

+

+

+

Hydrolysis of

      

Starch

+

+

+

Gelatine

+

+

+

Casein

+

+

+

+

+

Utilization of citrate

+

+

ND

Formation of dihydroxyacetone

+

  1. Abbreviations: + positive reaction, − negative reaction, ND not determined. The phenotyping characteristics of the type strains B. subtilis ATCC6051 [40], G. toebii SK-1 [40, 41] and A. flavithermus DSM 2641 [42, 43] were used as references.