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Table 1 Occurence of LspC3–41I system and the transformation capability of L. sphaericus

From: The LspC3–41I restriction-modification system is the major determinant for genetic manipulations of Lysinibacillus sphaericus C3–41

Strains

Serotypea

Sequence type (ST)b

PCR resultsc for

Transformation efficiency (cfu μg-1 plasmid DNA)

lspC3–41IR

lspC3–41 IM

Untreated

M. HaeIII

C3–41 CFE

Recipient CFE

C3–41

H5

1

+

+

0

2.2 × 105

2.4 × 105

2.4 × 105

2362

H5

1

+

+

0

1.1 × 105

4.6 × 104

4.2 × 104

1593

H5

1

+

+

0

1.9 × 105

7.0 × 104

5.5 × 104

47-6b

H6

2

-

-

0

0

0

0

2297

H25

4

-

-

3.6 × 104

ND

ND

ND

IAB872

H48

5

-

-

0

ND

0

0

IAB881

H3

6

-

-

0

ND

0

0

NRS1693

H2

7

-

-

7.4 × 105

ND

ND

ND

2314-2

H9

8

-

-

0

ND

0

0

LP1-G

H3

9

-

-

0

0

0

0

Bs-197

H1

10

-

-

7

ND

8

7

2173

H26

11

-

-

0

0

0

0

Cok31

H9

12

-

-

0

ND

0

0

SSII-1

H2

13

-

-

0

ND

0

0

KellenQ

H1

14

-

-

0

ND

0

0

Dak614

H4

15

-

-

0

ND

0

0

  1. aSerotype is as determined by [2]
  2. bST is as determined by [9]
  3. c+, present; -, absent
  4. ND means not determined