Fig. 3From: Transposon insertion sequencing reveals T4SS as the major genetic trait for conjugation transfer of multi-drug resistance pEIB202 from Edwardsiella TIS identification and verification of genes associated with pEIB202 transfer. a Artemis screenshot of abundance of reads in topA, p007 and T4SS genes in input (red) and output (green) samples. The height of the red and green bars correlates with the number of reads. b The transfer frequency of pEIB202 from each strain to ∆P::pNQ705K. Strains were conjugated at 30 °C for 8 h. c qRT-PCR analysis of the expression of T4SS genes in each strain grown in LB. * P < 0.01, ** P < 0.001, ***P < 0.0001, student’s t-testBack to article page