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Table 5 List of proteins identified to interact with DprA or PilG by pull-down experiments with co-immunoprecipitation (Co-IP) and mass spectrometry (MS)

From: Comparative proteomic analysis of Neisseria meningitidis wildtype and dprA null mutant strains links DNA processing to pilus biogenesis

Protein IDs

Protein names

Gene names

Co-IP and MS identified proteins that interact with DprA, PilG, and/or PilE

Anti-DprA-Ab

Anti-PilG-Ab

Anti-PilE-Ab

Wt

ΔdprA

Wt

ΔpilG

Wt

Q9K1K1

DNA processing chain A

dprA

+

Q7DDR1

Pilus assembly protein

pilG

+

+

+

+

P05431 Q4W588 Q4W587 Q4W586 Q4W589 Q4W585 Q4W590 Q4W592

Fimbrial protein; PilS cassette

pilE & pilS

+

+

+

+

+

Q4W563

PilN protein

pilN

+

+

+

+

+

Q70M91

Type IV pilus biogenesis and competence protein PilQ

pilQ

+

+

+

Q7DD78

PilO protein

pilO

+

Q9JY02

PilM protein

pilM

+

Q7DDU1

Twitching motility protein

pilT-1

+

+

P0A0S6

Cell division protein

ftsZ

+

+

Q9K0X8

Cell division protein

ftsA

+

Q7DDS7

Septum site-determining protein

minD

+

+

Q9JXD7

Probable malate:quinone oxidoreductase

mqo

+

+

Q7DDL4

Glutaredoxin

NMB0773

+

P0DH59

Protein RecA

recA

+

+

+

P66849

Single-stranded DNA-binding protein

ssb

+

+

+

+

Q9JZ92

Recombination protein

recR

+

Q9K0W0

DNA repair protein

recO

+

Q9K1K0

DNA topoisomerase 1

topA

+

  1. Most of these proteins were also revealed to be significantly less abundant in the Nm ΔdprA mutant as compared to wildtype (wt) by MS of whole cell lysates. In Co-IP, DprA, PilG, or PilE was used as bait proteins through their reactivity with specific rabbit polyclonal antibodies (Ab). In order for DprA, PilG or PilE to target their interacting proteins, the Ab against the bait proteins were incubated with the cell lysates from Nm wt as well as the cell lysates from the Nm ΔdprA or ΔpilG mutant (in the mutant the bait protein is absent), and thereby the Ab binds the bait protein. The bait protein coupled with the antibody binds its interacting partner, forming the antibody-bait-prey protein complex. The “+” sign designates the formation of antibody-bait-prey protein complex whereas the “-” sign designates the absence of complex formation/interaction