Fig. 6From: Comparative proteomic analysis of Neisseria meningitidis wildtype and dprA null mutant strains links DNA processing to pilus biogenesisPilG and DprA immunoblots. MC58 wt and ΔdprA mutant overnight cultures were harvested in 1× PBS and heat inactivated at 600C for 30 min. The cells were disrupted using MagNa Lyser (6× 90s at a speed of 6000 rpm). The cell lysates were prepared in Laemmli sample buffer and SDS-PAGE was run. The proteins transferred onto PVDF membrane. The membranes were incubated with ati-PilG (a) and ati-DprA (b) rabbit polyclonal primary antibodies, and anti-rabbit-IgG-horseradish peroxidase conjugate secondary antibody. The immunoblots were developed with Immun-Star WesternC Chemiluminescent kit (Bio-Rad) and visualized using a ChemiDoc Touch imager (Bio-Rad). Results were analysed using the Image Lab software (Bio-Rad)Back to article page