Application of hierarchical oligonucleotide primer extension (HOPE) to assess relative abundances of ammonia- and nitrite-oxidizing bacteria

Table 3 Water quality for the influent, effluent and chlorinated effluent samples collected from the wastewater treatment plant and the corresponding relative abundances of AOB and NOB as quantified using the HOPE and amplicon sequencing approach

	Total nitrogen, TN (mg/L)	NH₄ ⁺-N (mg/L)	NO₃ ^--N (mg/L)	Nse1472	Nsv443	Ntspa712	Ntspa572	Nit1000	Nitrosomonas	Nitrosospira	Nitrobacter	Nitrospira
	Total nitrogen, TN (mg/L)	NH₄ ⁺-N (mg/L)	NO₃ ^--N (mg/L)	Relative abundance (%) with respect to Eub338Ia (HOPE data)					Relative abundance (%) with respect to total bacteria (Amplicon sequencing data)
Influent	17 ± 1.3	12.3 ± 2.4	2.9 ± 0.8	0.28 ± 0.43	Not detected	3.1 ± 1.0	0.6 ± 0.5	Not detected	Not detected	Not detected	Not detected	0.02 ± 0.04
Effluent	17 ± 1.4	Not applicable	14 ± 2.3	0.61 ± 0.73	1.1 ± 2.9	2.3 ± 1.7	2.0 ± 1.6	Not detected	0.004 ± 0.007	0.008 ± 0.014	Not detected	1.8 ± 2.6
Chlorinated effluent	14 ± 2.4	Not applicable	8.6 ± 0.9	0.03 ± 0.07	0.2 ± 0.5	1.0 ± 1.3	1.6 ± 2.2	Not detected	0.01 ± 0.02	0.003 ± 0.008	Not detected	1.0 ± 1.5
Oxic sludge	Not applicable			0.23 ± 0.13	0.21 ± 0.28	8.1 ± 3.4	4.8 ± 2.4	0.17 ± 0.21	Not detected	Not detected	Not detected	6.4 ± 1.7

ISSN: 1471-2180