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Table 2 Efficiency of P1 transduction: complementation by different pnp mutant alleles

From: Polynucleotide phosphorylase is implicated in homologous recombination and DNA repair in Escherichia coli

Plasmida

pnp allele on plasmid

trpE

leuA

TFb (Ă—107)

TF fractionc

TFb (Ă—107)

TF fractionc

pAZ101

wt

2.53 (±0.09)

1.00

4.40 (±0.35)

1.00

pGZ119HE

none

0.75 (±0.20)

0.30

1.34 (±0.30)

0.30

pAZ1112

S438A

2.07 (±0.52)

0.82

2.70 (±0.46)

0.60

pAZ1113

ΔKH

0.55 (±0.15)

0.22

1.18 (±0.24)

0.26

pAZ1114

ΔS1

0.53 (±0.19)

0.21

1.19 (±0.25)

0.27

pAZ133

ΔKH-S1

0.06 (±0.02)

0.02

0.17 (±0.06)

0.04

  1. ain C-5691 (Δpnp) strain
  2. btransduction frequency (TF) is the ratio of transductants to adsorbed phage
  3. cratio to TF of wild type strain