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Fig. 3 | BMC Microbiology

Fig. 3

From: Polynucleotide phosphorylase is implicated in homologous recombination and DNA repair in Escherichia coli

Fig. 3

Template-independent synthesis of ssDNA or RNA catalyzed by different purified His-tagged PNPases. [32P]-5'-end labeled RNA (RNA01; panel a) or ssDNA (DNA02; panel b) 20-mers, 4 nM each, were incubated at 26 °C with purified His-tagged PNPase or PNPase mutants in the RBDs (10 nM each) in buffer a with 100 μM ADP (Panel a) or 100 μM dADP (for ssDNA Panel b) for the times indicated. The reaction products were fractionated by denaturing 6% PAGE. The oligonucleotide band intensities were evaluated by ImageQuant, normalized to the intensity of the 0 min sample, and plotted versus the time. Data from panels a and b are shown in panels c and d, respectively

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BMC Microbiology

ISSN: 1471-2180

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