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Fig. 1 | BMC Microbiology

Fig. 1

From: Polynucleotide phosphorylase is implicated in homologous recombination and DNA repair in Escherichia coli

Fig. 1

Survival of E. coli pnp and rnb mutants to chronic zeocin treatment. Bacterial cultures grown overnight at 37 °C in LD broth with chloramphenicol were serially diluted and plated onto LD-agar with chloramphenicol and 0, 4, 8, 12, 16, 20 μg/ml zeocin; the plates were incubated at 37 °C for 2 days. The surviving fraction was calculated as the ratio of colony forming units (CFUs) at different zeocin concentration to the untreated control. The means of at least two independent experiments and the standard deviations (error bars) are shown. Zeocin inhibitory concentration giving 1% survival (IC1) was extrapolated from the survival curves and reported as μg/ml on the right column in the panel legends. a Complementation of E. coli pnp deletion mutant with different pnp alleles. b Complementation of E. coli pnp and rnb deletion mutants. The pnp or rnb alleles harbored by the host chromosome and or by the plasmids are indicated by the labels before and after the slash, respectively. Bacterial strains: wt, C-1a; Δpnp, C-5691; Δrnb, C-5981. Plasmids: −, pGZ119HE; pnp+, pAZ101; rnb+, pAZ1115; pnpΔKH, pAZ1113; pnpΔS1, pAZ1114; pnpΔKH-S1, pAZ133; pnpS438A, pAZ1112

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