Fig. 4
From: Detailed analysis of c-di-GMP mediated regulation of csgD expression in Salmonella typhimurium
The GGDEF-EAL domain protein STM3388 promotes csgD expression through its diguanylate cyclase activity, whereas the GGDEF-EAL domain protein STM1703 suppresses csgD expression through its phosphodiesterase activity. a Rdar morphotype and CsgD levels upon expression of wild type STM3388 and catalytic GGDEF and EAL mutants of STM3388 in STM3388 deletion mutant of S. typhimurium UMR1. b Rdar morphotype and CsgD levels upon overexpression of STM1703 and catalytic mutants of STM1703 in the STM1703 deletion background of S. typhimurium UMR1. Cells were grown for 24 h at 28°C on LB without salt agar plates supplemented with ampicillin (100 μg ml−1) and 0.1% L-arabinose. VC = Vector control pBAD30