Fig. 5

ChMob2 interacts with ChCbk1. Whole cell extracts from strains expressing different combinations of ChMob2-GFP and ChCbk1-HA were used to analyze the interaction of ChMob2 and ChCbk1 by immunoprecipitation. Strains used from left to right: ΔChcbk1:: CBK1-HA pTef1a-MOB2-GFP (CY6681), the parental strain ΔChku80 (CY6021), ΔChcbk1:: CBK1-HA (CY6678) and pTef1a-MOB2-GFP (CY6543). Western blots of whole cell protein extracts (input), immunoprecipitates with anti-HA antibody coated beads (IP: α-HA) and immunoprecipitates with anti-GFP antibody coated beads (IP: α-GFP) were decorated either with anti-GFP (upper panels) or with anti-HA (lower panels) primary antibodies. The band corresponding to Mob2-GFP is marked with an arrow. The multiple bands corresponding to Cbk1-HA are marked with a bracket. Asterisks mark bands corresponding to primary antibody which are present in the IP and therefore are recognized by the secondary antibody