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Fig. 2 | BMC Microbiology

Fig. 2

From: ChMob2 binds to ChCbk1 and promotes virulence and conidiation of the fungal pathogen Colletotrichum higginsianum

Fig. 2

vir-88 encodes a hypomorphic ChMOB2 allele. a Schematic representation of the ChMOB2 locus in wild-type (top) and vir-88 (bottom). Beginning and end of the ChMOB2 transcript are shown relative to the start codon. Introns are depicted as striped boxes. LB = left border; RB = right border. b Quantitative RT-PCR of ChMOB2 mRNA abundance in conidia, appressoria, mycelium and in infected plants (4 dpi; in this case only data from WT is shown). The data were normalized against α-TUBULIN (CH063_01222) and are shown as the average of three biological replicates. c Semi-quantitative RT-PCR of ChMOB2 and α-TUBULIN in RNA samples from in vitro appressoria 0, 4, 8 and 12 h after inoculation. cDNAs from WT, vir-88 and ΔChace2 (CY6353, see below) strains were used as template for PCR. Genomic WT DNA (gDNA) or H2O were used as controls. d, e A. thaliana leaves at 3 days after infection with WT, vir-88 and vir-88 strains complemented with either the ChMOB2 wild-type allele (pMOB2, pCK3110) or with a pTef-MOB2-GFP construct (pCK4129). Typical trypan blue stained leaves are shown in d. Quantifications are shown in e. Scale bars = 20 μm. f Average volume of conidia from the indicated strains recovered from oatmeal agar after 7 days. The data from 3 separate plates is given. Error bars are represented as the standard error from three replicates. Significant differences based on t-tests (p < 0.05) are marked with asterisks

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