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Table 1 DNA yield when using the modified DNA extraction protocol

From: Optimisation of methods for bacterial skin microbiome investigation: primer selection and comparison of the 454 versus MiSeq platform

No

Bacterial Strain

Gram stain

Yield

ng/μl

1

Pseudomonas aeruginosa–PA01

Negative

17

2

Bacillus subtilus–NCTC 831

Positive

34.1

3

Escherichia coli–NCTC 8809

Negative

>60

4

Listeria monocytogenes–NCTC 2166

Positive

28.7

5

Mycobacterium phlei–NCTC 8151

 

>60

6

Staphylococcus aureus–NCTC 7447

Positive

36.2

7

Enterococcus faecalis NCTC 370

Positive

40.4

8

Staphylococcus epidermidis Rp62a

Positive

8.68

9

Bordetella bronchiseptica–253

Positive

>60

10

Peptoclostridium difficile–630

Positive

8

11

Staphylococcus saprophyticus–NCTC 7687

Positive

23.1