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Table 1 Comparison of serological diagnosis by the novel criteria of ELISA and the stanndard criteria of IF assay

From: Evaluation of a broad-ranging and convenient enzyme-linked immunosorbent assay using the lysate of infected cells with five serotypes of Orientia tsutsugamushi, a causative agent of scrub typhus

Patients

 

No1

No2

No3

No4

No5

No6

No7

No8

No9

No10

No11

No12

No13

No14

No15

No16

No17

No18

No19

ELISA

IgM > Av + 3SD 1)

+

+

+

+

+

+

+

+

+

+

-

+

+

+

+

+

+

+

+

Eelevation of antibody between paired sera 2)

+

+

+

+

+

+

-

+

+

+

+

-

+

+

-

+

-

+

+

Diagonosis

P 3)

P

P

P

P

P

P

P

P

P

P

P

P

P

P

P

P

P

P

IF

IgM ≥80

+

+

+

+

+

+

+

+

+

+

-

+

+

-

+

+

+

+

+

Eelevation of antibody between paired sera

+

+

+

+

+

+

-

+

-

+

+

-

+

+

-

+

+

+

+

Diagonosis

P

P

P

P

P

P

P

P

P

P

P

P

P

P

P

P

P

P

P

  1. 1) IgM antibody elevation in acutephase sera, 2) ≥4 or more folds-elevation of antibody titer between paired sera, 3) P:positive