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Fig. 2 | BMC Microbiology

Fig. 2

From: N-acetyl-cysteine exhibits potent anti-mycobacterial activity in addition to its known anti-oxidative functions

Fig. 2

NAC restrains mycobacterial growth within THP-1 macrophages and exhibits a direct anti-mycobacterial effect on extracellular bacteria in vitro. a Human-THP-1 macrophages were infected with M. tuberculosis, M. avium or M. bovis strains at an MOI of 10 for 3 h. Extracellular bacteria were removed by washing. Cells were then cultivated for 5 days in the presence of NAC at 10 mM. CFU counts were assessed as described in Methods. b and c Mycobacteria strains were grown in Middlebrook 7H9 supplemented with OADC in 96-well plates. Metabolic activity measurements (b) and CFU counts (c) were performed as described in Methods. Significant differences were observed for the indicated experimental conditions compared to untreated cultures (*p < 0.05; **p < 0.01, ***p < 0.001). The data represent the means ± SEM of triplicate samples. The data shown are representative of three independent experiments

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