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Fig. 2 | BMC Microbiology

Fig. 2

From: Characterization of pneumococcal Ser/Thr protein phosphatase phpP mutant and identification of a novel PhpP substrate, putative RNA binding protein Jag

Fig. 2

Phenotypic characterization of phpP knock-out strain. a Growth curves of WT (Sp1), ΔstkP (Sp10), and ΔphpP (Sp113) strains cultivated statically at 37 °C in TSB medium. b Heat stress. Growth curves of the WT and both mutant strains in TSB medium were measured at 37 and 40 °C. c Oxidative stress. The liquid cultures of the WT, ΔstkP and ΔphpP strain were exposed to different concentration of H2O2 and aliquots of cultures were plated to evaluate survival upon oxidative stress. Number of CFU (colony forming units) at non-stress conditions (0 mM H2O2) were set as 100 %. d Induced competence. Transformation efficiency of WT, ΔphpP and ΔstkP strain grown in BHI medium upon addition of CSP. e, f, g Natural competence. Natural competence is expressed as % of viable transformants of WT (e), ΔphpP (f) and ΔstkP (g) strain during cultivation in BHI medium (OD600nm) without addition of CSP. All data shown represent mean ± SD for three independent experiments. Where error bars are not shown, the SD was within the size of the symbol. All growth curves are plotted in semi logarithmic scale

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