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Fig. 4 | BMC Microbiology

Fig. 4

From: Isolation, identification and characterization of Paenibacillus polymyxa CR1 with potentials for biopesticide, biofertilization, biomass degradation and biofuel production

Fig. 4

Nitrogen-fixation by P. polymyxa CR1. P. polymyxa CR1 inoculated on nitrogen free media (NFM) was assessed for growth in reference to non-nitrogen fixing E. coli O157:H7. a NFM medium; b NFM supplemented with 5 mM NH4Cl; c The presence of nitrogen fixing genes. Primer pairs PolF and PolR were utilized to amplify 352 bp of the nifH gene from four single colonies of the same P. polymyxa CR1 strain (lanes 2–5), or in a PCR reaction control without DNA (lane 1). Presence of a nif gene cluster was confirmed by DNA sequencing

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