Skip to main content
Fig. 3 | BMC Microbiology

Fig. 3

From: DNA-based culture-independent analysis detects the presence of group a streptococcus in throat samples from healthy adults in Japan

Fig. 3

Primer sensitivity. Representative data for the detection limit of GAS-specific primers using MightyAmp DNA polymerase. S. pyogenes JRS4 DNA was serially diluted (6.25 ng to 0.625 fg DNA corresponds to 3 × 106 to 0.3 genomic copies of GAS). Sterile deionized water was used for the negative control. Lane M, DNA marker; 1, 6.25 ng; 2, 625 pg; 3, 62.5 pg; 4, 6.25 pg; 5, 625 fg; 6, 62.5 fg; 7, 6.25 fg; 8, 0.625 fg; N, negative control. Arrows indicate the detection limits of the template DNA concentration

Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us