Fig. 3

Difference of ciprofloxacin and biotin limitation after yggB deletion. The strains MB001 (black) and MB001∆yggB (white) were cultured to an optical density of 2 to 5 in CGXII supplemented with 1 % (w/v) glucose and ciprofloxacin was applied. In addition, the MB001 strain (black) was compared to MB001∆yggB (white) during biotin limitation. Therefore the pre-limited cells (in CGXII, 4 % glucose, 0 μg biotin per L) were re-inocculated to CGXII, containing 2 μg biotin per L, supplemented with 1 % (w/v) glucose. The glutamate concentration of both conditions was determined in the culture supernatant after the complete consumption of glucose. Values and error bars represent the mean and the standard error of triplicate cultivations