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Fig. 2 | BMC Microbiology

Fig. 2

From: Cobalamin production by Lactobacillus coryniformis: biochemical identification of the synthetized corrinoid and genomic analysis of the biosynthetic cluster

Fig. 2

Liquid chromatography–electrospray ionization/tandem mass spectrometry (LC/ESI–MS/MS) chromatograms of peaks with cobalamin activity from L. coryniformis CRL 1001. The transitions are shown together (SRMs, 672.5 m/z -- > 136.0 m/z calculated for adenine y 672.5 m/z -- > 348.0 m/z correspond to the lower ligand in which adenine is the aglycon attached to ribofuranose 3-phosphate). Total ion chromatogram (TIC) of the two active peaks, RT 23.77 min (a) and RT 24.20 min (b) and MS/MS spectrum from m/z 660 to 685 of the peak with RT 8.142 min with MS/MS spectrum showing the transitions

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