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Fig. 1 | BMC Microbiology

Fig. 1

From: In vivo functional and molecular characterization of the Penicillin-Binding Protein 4 (DacB) of Pseudomonas aeruginosa

Fig. 1

a Purification of LMM-PBP4. SDS-PAGE analysis of purified recombinant proteins PBP4HNC (lanes 2, 3, 4, 5), PBP4HN (lanes 6, 7, 8, 9) and PBP4HC (lanes 10, 11, 12, 13). Lane 1, molecular weight marker (51 kDa); lanes 2, 6 and 10, flow-through; lanes 3, 7 and 11, elution with 125 mM imidazole from Ni-NTA; lanes 4, 8 and 12, elution with 250 mM imidazole from Ni-NTA; lanes 5, 9 and 13, elution with 500 mM imidazole from Ni-NTA.. b Identification assays for LMM-PBP4. Pattern of Bocillin™ FL binding to membrane protein extracts prepared from strains E. coli BL21(DE3)/pET-PBP4HNC, E. coli BL21(DE3)/pET-PBP4HN and E. coli BL21(DE3)/pET-PBP4HC. Extracts from non induced cell (NI) and induced by IPTG (IPTG) are shown. Unprocessed and mature forms of the overexpressed proteins are indicated by arrows. PBPs profile model (PBP) for Escherichia coli is shown

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BMC Microbiology

ISSN: 1471-2180

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