Enterococcus hirae LcpA (Psr), a new peptidoglycan-binding protein localized at the division site

BMC Microbiology

Table 2 Primers used in this study

Primer	Sequence (5′ to 3′)	Application
32	ctttaagaaggagatatacatatgAAAACATTTCAAAAAGTGATTTTAGGTCTATTG	Cloning lcpA in pET-22b
33^a	gtggtggtggtggtgTTGGTCTAAAAACTGATTGATTGCTAATTG	Cloning lcpA and lcpA _30–293 in pET-22b
43	ctttaagaaggagatatacatatgTCTTCAACGAGTCGAGCAGACGATGTA	Cloning lcpA _30–293 in pET-22b
CF9	CATGCCATGGCAAAAACATTTCAAAAAGTGATTTTAGGTCTATTG	Cloning lcpA in pCIPblaM
CF42^a	CGAGCTCTTGGTCTAAAAACTGATTGATTG	Cloning lcpA in pCIPblaM

Reverse primers are indicated by ^a. Underlined sequences are restriction sites engineered for cloning. Bases noted in small letters are homologous to bases of the linearized vector when using In-Fusion® HD Cloning Kit

ISSN: 1471-2180