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Fig. 7 | BMC Microbiology

Fig. 7

From: Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling-mediated mitochondria pathway

Fig. 7

Induction of the expression of caspase-3/9 in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes of the expression of caspases was assessed using an immunoblotting assay. a Representative images of immunoblots of indicated caspases or their activated forms, revealed an enhanced activities of caspase 3 and 9 in airway epithelial cells infected with MO, and an addition of MEK/ERK signalling PD980025 or ROS scavenger NAC resulted in reducing the MO-induced activities of these caspases. b Semi-quantitative analysis of the expression of proteins in (a) by evaluating the relative densitometric densities using arbitrary units (A.U.), calculated based on the densitometric signal of a protein of interest over that of the corresponding β-actin internal control

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