Skip to main content
Fig. 4 | BMC Microbiology

Fig. 4

From: Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling-mediated mitochondria pathway

Fig. 4

The production of ROS and expression of MEK/ERK signalling in sheep airway epithelial cells induced by M. ovipneumoniae infection. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, and the production of ROS was measured by a fluorometrical assay using DCFH-DA dye (a), and changes in the expression of key molecules of the MEK/ERK signalling pathway was assessed using an immunoblotting method. a An infection of MO led an increased production of ROS in airway epithelial cells, which could be partially inhibited by MEK/ERK signalling inhibitor PD980025. b An immunoblotting assay revealed an evoked expression of RAS, RAF, p-RAF, MEK, p-MEK, ERK and p-ERK proteins in cells infected with MO, and this induction was inhibited PD980025 and ROS scavenger NAC. c Semi-quantitative analysis of the expression of proteins in (b) by evaluating the relative densitometric densities using arbitrary units (A.U.), calculated according to the densitometric signal of a protein of interest over that of the corresponding β-actin internal control. Data were expressed as the mean ± SD from three independent experiments. Compared to the uninfected controls, *: p < 0.05; **: p < 0.01 in cells untreated with PD980025. The cell numbers of each transwell with diameter of 24 mm was determined as 107

Back to article page