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Fig. 10 | BMC Microbiology

Fig. 10

From: Structure-function analysis of Avian β-defensin-6 and β-defensin-12: role of charge and disulfide bridges

Fig. 10

Transmission electron microscopy (TEM) analysis of AvBD-induced morphological changes of S. Typhimurium. Treatment of bacteria with wild-type and reduced AvBDs at 37 °C for 30 min resulted in fuzzy membrane (1), vacuole formation (2), membrane bleb (3), morphological change (4), and cytoplasm membrane shrinking (5). Bacteria were treated with AvBD-6 (a1-3), AvBD-12 (b1-3), reduced AvBD-6 (c), and reduced AvBD-12 (d). Untreated bacteria showed intact membrane, uniform cytoplasm (e). Figures are representatives of 10 images per treatment group. Approximately 29 % of bacteria treated with wild type AvBDs and 27 % of bacteria treated with reduced AvBDs displayed ultrastructural changes. Scale bar: 1 μm

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