Fig. 4
From: A method for assessing efficiency of bacterial cell disruption and DNA release
Schema showing use of the acid/HPLC method to determine DNA extraction efficiency by different methods. Equal aliquots of a bacterial suspension are centrifuged and the washed pellets are treated with standard acid/alkali or subjected to an extraction method (e.g., method #1. method #2, etc.). The extract is then treated by acid/alkali. The amount of adenine in the extract is compared to the amount of adenine from the pellet of intact cells. The efficiency of DNA extraction is given by the ratio of adenine (in extract) divided by adenine (from intact cells)