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Fig. 2 | BMC Microbiology

Fig. 2

From: A method for assessing efficiency of bacterial cell disruption and DNA release

Fig. 2

Comparison of the amount of DNA in intact cells, as detected by acid/HPLC, with the amount of DNA released after different disruption methods. Disruption methods used were lysozyme (Lys) for B.vegetative cells and bead-beating (BB) for B. subtilis spores and M. smegmatis. In each experiment, an aliquot of a cell suspension(6x108 B. subtilis vegetative or 8x108 B. subtilis spores, or 2x109 cells of M. smegmatis) was treated either by (i) the standard acid/alkali method (HCl) or (ii) disrupted as indicated. After disruption, lysates were centrifuged to remove debris, then treated by the standard acid/alkali method to release adenine from the released DNA. The amount of adenine was determined by HPLC. The mean and range of duplicate samples are shown. The right-hand axis refers to the spores samples. Adenine (AUC), Area Under the Curve for adenine in milliabsorbance units

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