Fig. 4

SDS-PAGE and western blot analysis of purified rCtE-MOMP. a SDS-PAGE; (b) anti-CtE EB mouse sera western blot; (c) anti-E.coli control nOMV (native outer membrane vesicle from E. coli that does not contain recombinant MOMP gene) mouse sera; (d) anti-E.coli LPS monoclonal antibody. Sample 1, control nCtE-MOMP; sample 2, purified rCtE-MOMP; sample 3, E. coli whole cell lysate; all samples were heated and reduced. Monomeric and dimeric forms of MOMP, as well as E. coli LPS are indicated