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Fig. 3 | BMC Microbiology

Fig. 3

From: Recombinant expression of Chlamydia trachomatis major outer membrane protein in E. Coli outer membrane as a substrate for vaccine research

Fig. 3

Expression of rCtE-MOMP on E. coli outer membrane under optimized expression conditions. An E. coli transformant expressing rCtE-MOMP with a PelB leader constructed in a pAVE029 vector was grown in Cinnabar medium at 37 °C and induced by 1 mM IPTG at 30 °C for 4 h when OD590 reaches ~0.5. Whole cell flow cytometry antibody binding histograms were shown: red, anti-CtE EB mouse sera and blue, a negative control antibody. Geomean fluorescence intensities were also shown in table

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