Fig. 3From: Recombinant expression of Chlamydia trachomatis major outer membrane protein in E. Coli outer membrane as a substrate for vaccine researchExpression of rCtE-MOMP on E. coli outer membrane under optimized expression conditions. An E. coli transformant expressing rCtE-MOMP with a PelB leader constructed in a pAVE029 vector was grown in Cinnabar medium at 37 °C and induced by 1 mM IPTG at 30 °C for 4 h when OD590 reaches ~0.5. Whole cell flow cytometry antibody binding histograms were shown: red, anti-CtE EB mouse sera and blue, a negative control antibody. Geomean fluorescence intensities were also shown in tableBack to article page