Table 3 Characteristic of mutations found in resistant isolates to RMP (n = 23), PZA (n = 48) or EMB (n = 14) compared to susceptible strains (n = 32)
From: Genotypic characterization of drug resistant Mycobacterium tuberculosis in Quebec, 2002-2012
Drug resistance | Genes | No. of isolates | Changes | |
|---|---|---|---|---|
RMPR | rpoB | 23 | Nucleotides | Amino acid |
Single mutations | ||||
1 | TTC514 | Ins F514 | ||
2 | GAC/GTC | D516V | ||
4 | CAC/GAC | H526D | ||
2 | CAC/CGC | H526R | ||
8 | TCG/TTG | S531L | ||
1 | TCG/TTT | S531F | ||
1 | CTG/CCG | L533P | ||
Multiple mutations | ||||
2 | CTG/CGG, GAC/TAC | L511R, D516Y | ||
2 | TCG/TTG, ACC/ATG | S531L, T480I | ||
RMPS | rpoB | 32 | WT | None |
PZAR | pncA | 48 | ||
Single mutations | ||||
1 | -11A/G | None | ||
1 | CTG/CCG | L27P | ||
1 | Del G164 | Frameshifta | ||
2 | ATC/AGC | I90S | ||
1 | GCG/CCG | A92Pa | ||
1 | Del 9 bp 380 | Del D,E,Va | ||
1 | ACC/CCC | T135P | ||
1 | Ins AT 447 | Frameshifta | ||
1 | ACC/CCC | T168P | ||
Multiple mutations | ||||
35 | Del8 bp446, CGC/AGC | Frameshift, R140S | ||
1 | GCA/GCG | A46A | ||
2 | WT | None | ||
PZAS | pncA | 32 | WT | None |
EMBR | embB | 14 | ||
Single mutations | ||||
4 | ATG/ATA | M306I | ||
3 | ATG/GTG | M306V | ||
2 | TAT/TCT | Y319S | ||
1 | GAT/TAT | D328Y | ||
1 | GAC/GCC | D354A | ||
1 | GGC/AGC | G406S | ||
Multiple mutations | ||||
1 | ATG/ATA, CTG/CTA, GAG/GCG | M306I, L355L, E387A | ||
1 | WT | None | ||
EMBS | embB | 32 | WT | None |