Fig. 2

Excretion of malate (circles) and fumarate (squares) from dme (filled) and tme (open) mutant strains. Strains grown overnight in M9-glucose plus succinate were transferred into modified M9 containing 2.5 mM phosphate and 5 mM succinate. Samples were taken 1, 2 and 3.5 h post transfer and the culture supernatants were analyzed by GC-MS (see methods). The relative response factors for fumarate and malate relative to the standard ribitol were determined. Error bars were calculated using standard deviation of the means for three experimental replicates