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Fig. 1 | BMC Microbiology

Fig. 1

From: Loss of malic enzymes leads to metabolic imbalance and altered levels of trehalose and putrescine in the bacterium Sinorhizobium meliloti

Fig. 1

Relative response factors (RRF) for intracellular metabolites with significantly different RRFs (P values of < 0.05 in ANOVA) from the wild-type strain, dme, tme and dme tme double mutants. Note the different RRF scale for the three growth conditions. Metabolites were 6-phospho-sugars (likely fructose-6-phosphate (6PS1), mannose-6-phosphate (6PS2) and glucose-6-phosphate (6PS3)), putrescine and trehalose. Strains were grown in M9-Succinate, M9-Glucose, M9-Succinate plus Glucose. The trehalose RRFs were negligible in extracts for strains grown in M9-glucose. For M9-Succinate + Glucose, the cells grown in Succinate + Glucose were washed and incubated for 2 h in modified M9-Succinate. Error bars were calculated using standard deviation of the mean from three independent cultures

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