Fig. 1From: Loss of malic enzymes leads to metabolic imbalance and altered levels of trehalose and putrescine in the bacterium Sinorhizobium meliloti Relative response factors (RRF) for intracellular metabolites with significantly different RRFs (P values of < 0.05 in ANOVA) from the wild-type strain, dme, tme and dme tme double mutants. Note the different RRF scale for the three growth conditions. Metabolites were 6-phospho-sugars (likely fructose-6-phosphate (6PS1), mannose-6-phosphate (6PS2) and glucose-6-phosphate (6PS3)), putrescine and trehalose. Strains were grown in M9-Succinate, M9-Glucose, M9-Succinate plus Glucose. The trehalose RRFs were negligible in extracts for strains grown in M9-glucose. For M9-Succinate + Glucose, the cells grown in Succinate + Glucose were washed and incubated for 2 h in modified M9-Succinate. Error bars were calculated using standard deviation of the mean from three independent culturesBack to article page