Investigation of availability of a high throughput screening method for predicting butanol solvent -producing ability of Clostridium beijerinckii

Table 1 Performance (the highest value) of ABE fermentation products of selected mutants and the parental strain using soluble starch by batch fermentation

Parameters	8052	M8A	M17A	M27A	M31B	M38B	M47B	M12C	M34C	M53C
But. (g/L)	3.24^a ±0.45	5.12^b ±0.36	6.03 ^b ±0.27	5.23^b ±0.47	8.89^c ±0.39	9.26^c ±0.57	10.23^c ±0.62	13.76^d ±0.73	12.34^d ±0.69	13.46^d ± 0.85
Ace.(g/L)	1.11 ± 0.15	2.36 ± 0.34	2.11 ± 0.26	2.59 ± 0.43	3.68 ± 0.37	3.56 ± 0.28	3.51 ± 0.65	5.51 ± 0.43	5.11 ± 0.24	5.76 ± 0.37
Eth. (g/L)	0.58 ± 0.18	0.88 ± 0.25	1.12 ± 0.41	1.13 ± 0.31	1.52 ± 0.35	1.93 ± 0.31	1.68 ± 0.37	3.02 ± 0.45	3.33 ± 0.24	3.17 ± 0.53
Total ABE (g/L)	4.93	8.36	9.26	8.95	14.09	14.75	15.42	22.29	21.18	22.39
Total acids (g/L)	2.11	1.69	1.44	1.59	1.87	2.1	1.76	2.77	2.47	2.26
TS (g/L)	7.04	10.05	10.7	10.54	15.96	16.85	17.18	25.06	23.65	24.65
RCH (g/L)	30.72	14.32	20.44	18.44	11.34	12.34	13.22	7.62	7.17	8.47
ABE yield (g/g)	0.08	0.14	0.15	0.15	0.23	0.25	0.26	0.37	0.35	0.37
Product. (g/L.h)	0.05	0.08	0.10	0.09	0.15	0.15	0.16	0.23	0.22	0.23
But./TS(%)	46.02	50.95	56.35	49.62	55.70	54.63	59.54	54.90	52.17	54.60
FT(hours)	96	96	96	96	96		96	96	96

But butanol (g/L), Ace acetone (g/L), Eth ethanol (g/L), TS total solvents (g/L), RCH residual carbohydrate (g/L), Product productivity (g/L.h), But./TS butanol/total solvents (%), FT fermentation time (hours)
ABE yield (g/g): total ABE production divided by gross weight of soluble starch. Productivity (g/L.h): Total solvents divided by fermentation time. ^a,b,c.d: different letters in table indicate significant differences based on multiple comparisons (P < 0.05)
Mean values of three independent replicates each and standard deviations are shown

ISSN: 1471-2180