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Fig. 1 | BMC Microbiology

Fig. 1

From: Pet serine protease from enteroaggregative Escherichia coli stimulates the inflammatory response activating human macrophages

Fig. 1

Purification of Pet protein by FPLC. Pet protein was purified from supernatant of the minimal clone HB101(pCEFN1).a Culture supernatant ammonium sulfateprecipitated was passed through a Q-Sepharose and FPLC columns. b The toxin specificity was analyzed by Western blot with anti-pet antibody (1:500) and the reaction visualized with rabbit anti-IgG antibody conjugated with alkaline phosphatase (1:5000). The reaction was evidenced adding BCIP/NBT. Lines: MW markers; 1 Pet toxin; 2 Entamoeba histolytica HMI-IMSS antigen

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