Fig. 1From: Pet serine protease from enteroaggregative Escherichia coli stimulates the inflammatory response activating human macrophagesPurification of Pet protein by FPLC. Pet protein was purified from supernatant of the minimal clone HB101(pCEFN1).a Culture supernatant ammonium sulfateprecipitated was passed through a Q-Sepharose and FPLC columns. b The toxin specificity was analyzed by Western blot with anti-pet antibody (1:500) and the reaction visualized with rabbit anti-IgG antibody conjugated with alkaline phosphatase (1:5000). The reaction was evidenced adding BCIP/NBT. Lines: MW markers; 1 Pet toxin; 2 Entamoeba histolytica HMI-IMSS antigenBack to article page