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Fig. 5 | BMC Microbiology

Fig. 5

From: Immunoreactivity of the AAA+ chaperone ClpB from Leptospira interrogans with sera from Leptospira-infected animals

Fig. 5

Immune reactivity of the recombinant ClpBLi with bovine sera. a The purified ClpBLi protein (250 ng) was resolved by SDS-PAGE and analyzed by Western blotting using the antiserum against ClpBLi158–334 (a positive control; control +), polyclonal bovine antisera raised against: L. borgpetersenii serovar Hardjo (Leptospira-infected cattle), and sera collected from uninfected cattle (healthy group; negative control). The positions of protein size markers (M) (in kDa), PageRuler prestained Protein (Thermo Scientific), are shown on the left. The arrow indicates the position of ClpBLi (~100-kDa). b ELISA analysis of the recombinant ClpBLi protein as a capture antigen using the above bovine sera. Fetal bovine serum was also used. The data were analyzed using Welch adjusted one-way ANOVA. Symbols: (▫), the median value; (box), 25 %–75 % range around the median value, (whiskers), min-max range. (***) denotes P <... 0.001

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