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Fig. 4 | BMC Microbiology

Fig. 4

From: Immunoreactivity of the AAA+ chaperone ClpB from Leptospira interrogans with sera from Leptospira-infected animals

Fig. 4

Immune reactivity of the recombinant ClpBLi with rabbit sera. a The purified ClpBLi protein (250 ng) was resolved by SDS-PAGE and analyzed by Western blotting using: the antiserum against ClpBLi158–334 (a positive control), pre-immune control serum (a negative control), or polyclonal rabbit antisera raised against: L. interrogans and L. borgpetersenii serovars as indicated in the figure. The positions of protein size markers (M) (in kDa), PageRuler prestained Protein Ladder (Thermo Scientific), are shown on the left. The arrow indicates the position of ClpBLi. (b) ELISA analysis of the recombinant ClpBLi protein as a capture antigen using all the above rabbit sera. The data were analyzed using Welch adjusted one-way ANOVA. Symbols: (▫), the median value; (box), 25 %–75 % range around the median value; (whiskers), min-max range. (***) denotes P < 0.001; ns, not statistically significant

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