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Fig. 4 | BMC Microbiology

Fig. 4

From: E2 multimeric scaffold for vaccine formulation: immune response by intranasal delivery and transcriptome profile of E2-pulsed dendritic cells

Fig. 4

V3-specific CD8+ T-cell response. BALB/c mice (n = 3) were intranasally immunized with Env(V3)-E2 plus CT or E2wt particles. Isolated splenocytes were co-cultured with V3 (IGPGRAFYA311-318) peptide-pulsed γ-irradiated LPS-blasts; after 6 days of in vitro stimulation, effector cells were assayed for IFN-γ production by ICS. a Graph represents the mean (± S.D.) of percentage values of V3-specific CD8+ gated T cells secreting IFN-γ from all mice in each group. b Representative dot plot analysis of IFN-γ ICS from a single mouse in each group with the percentage values of IFN-γ positive cells indicated in the upper right corner of each square. Statistical significance was determined using the unpaired two-tailed Student's t-test; P value is reported. A representative experiment out of two is shown

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